Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method
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Check culture purity by making streak plates periodically and observing for a single species-characteristic type of colonies.
Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7. A standard strain of bacteria is used which is specific to the requirements of the material under test.
The test Method determines antibacterial activity of diffusable antimicrobial agents on treated textile materials. Individual reprints single or multiple copies of this method may be obtained by contacting AATCC at the above address or tel: A 50 TM Copyright? The average width of a zone of inhibition along a streak on either side of the test specimen may be calculated using the following equation: All OSHA standards and rules must also be consulted and followed.
Culture Medium mm length permits the specimens to lie across 5 parallel inoculum streaks each of diminishing width from about 8 mm to 4 mm wide. If you feel that your comments have not received a fair hearing, you should make your views known to Christopher S.
AATCC Test Method 147
After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. Precision and Bias 7.
AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but is cumbersome and time consuming for routine quality control and screening tests. In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise.
If no other bacterial species is specified, Staphylococcus aureus may be used as a representative Gram positive organism. Notes and References Check pH and adjust to 7. Avoid mutation by strict adherence to monthly stock transfers.
These safety precautions are for information purposes only. Avoid contamination by using good sterile technique in plating and transferring.
AATCC – Antimicrobial Fabric Test | Situ Biosciences LLC
Therefore, every mrthod and 5. Some of the bacteria used in this test are pathogenic; i. Safety Precautions reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment. Your comments will receive careful consideration at a meeting of the responsible technical committee, which you may attend.
Other recommended strains are listed below in Section 6. Report of results will include an observation of zones of inhibition and growth under the specimen aatcf present. Antibacterial Activity Assessment of Textile Materials: Your comments are invited either for revision of this test method or for additional methods and should be addressed to the AATCC Technical Center.
Mix well using appropriate agitation.
The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings. Parallel Streak Method Developed in by AATCC Committee RA31; reaffirmed msthod,; editorially revised,aatdc revisedwith title change; metohd revised and reaffirmed Foreword The Parallel Streak Method has filled a need for a relatively quick and easily executed qualitative method to determine antibacterial activity of diffusable antimicrobial agents on treated textile materials.
AATCC grants you a license as follows: AATCC takes no position respecting the validity of any patent rights asserted in connection with any item mentioned in this test method. To constitute acceptable antibacterial activity, there must be no bacterial colonies directly under the sample in the contact area. Wear safety glasses in all laboratory areas.